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Immunomodulatory profile of CM INF , CM INF -functionalized 3D scaffolds, and released media (CM R ) on activated PBMCs and Jurkat cells after 48 h of culture. Histograms representing PBMC proliferation (%) and NFAT activation in response to CM INF and 3D+CM INF ( A , B ) and the CM R obtained from 3D+CM INF collected at different time points ( C , D ). a Statistically significant vs. ( p < 0.05). b Statistically significant vs. CTR+ ( p < 0.05). c Statistically significant vs. CM R 6 h ( p < 0.05). d Statistically significant vs. CM R 24 h ( p < 0.05). e Statistically significant vs. CM R 48 h ( p < 0.05). f Statistically significant vs. CM R 72 h ( p < 0.05). g Statistically significant vs. CM R 7 d ( p < 0.05). ** and **** Statistically significant differences between groups ( p < 0.01 and 0.0001, respectively).

Journal: International Journal of Molecular Sciences

Article Title: Immuno-Instructive 3D Tendon Biomimetic Scaffolds Functionalized with Amniotic Epithelial Stem Cell Secretome for Controlled Inflammation and Targeted Macrophage Polarization

doi: 10.3390/ijms27042029

Figure Lengend Snippet: Immunomodulatory profile of CM INF , CM INF -functionalized 3D scaffolds, and released media (CM R ) on activated PBMCs and Jurkat cells after 48 h of culture. Histograms representing PBMC proliferation (%) and NFAT activation in response to CM INF and 3D+CM INF ( A , B ) and the CM R obtained from 3D+CM INF collected at different time points ( C , D ). a Statistically significant vs. ( p < 0.05). b Statistically significant vs. CTR+ ( p < 0.05). c Statistically significant vs. CM R 6 h ( p < 0.05). d Statistically significant vs. CM R 24 h ( p < 0.05). e Statistically significant vs. CM R 48 h ( p < 0.05). f Statistically significant vs. CM R 72 h ( p < 0.05). g Statistically significant vs. CM R 7 d ( p < 0.05). ** and **** Statistically significant differences between groups ( p < 0.01 and 0.0001, respectively).

Article Snippet: To investigate intracellular immune signaling related to cell proliferation, Jurkat-LuciaTM NFAT reporter cells (#jktl-nfat-cd28; InvivoGen, Toulouse, France) were used following previously established protocols [ , ] to assess NFAT-dependent T-cell activation via an NFAT-inducible Lucia luciferase construct.

Techniques: Activation Assay